LOCUS       40924_10961        4272 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCL476 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Love CA, Lilley PE, Dixon NE.
  TITLE     Stable high-copy-number bacteriophage lambda promoter vectors for 
            overproduction of proteins in Escherichia coli
  JOURNAL   Gene 176 (1-2), 49-53 (1996)
  PUBMED    8918231
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Yasuda S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2006) Contact:Seiichi Yasuda National Institute of
            Genetics, Microbial Genetics; Yata 1111, Mishima, Shizuoka 411-8540,
            Japan
REFERENCE   3  (bases 1 to 4272)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4272)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 176 
            (1-2), 49-53 (1996)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-FEB-2006) Contact:Seiichi Yasuda National Institute of Genetics,
            Microbial Genetics; Yata 1111, Mishima, Shizuoka 411-8540, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    53..419
                     /label=partition(pSC101)
                     /note="partition(pSC101)"
     promoter        474..578
                     /label=AmpR promoter
     CDS             579..1436
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1610..2198
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2486..2507
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2522..2552
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2560..2576
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2584..2600
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2859..3569)
                     /label=lambda repressor
                     /note="phage lambda repressor"
     regulatory      3617..3644
                     /label=lambda PR
                     /note="lambda PR"
                     /regulatory_class="promoter"
     RBS             3658..3666
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      3831..3859
                     /label=lambda PL
                     /note="lambda PL"
                     /regulatory_class="promoter"
     RBS             3986..3994
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4005..4022
                     /label=6xHis
                     /note="6xHis affinity tag"
     primer_bind     complement(4115..4131)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgataat tcgacagtaa
       61 gacgggtaag cctgttgatg ataccgctgc cttactgggt gcattagcca gtctgaatga
      121 cctgtcacgg gataatccga agtggtcaga ctggaaaatc agagggcagg aactgcgaac
      181 agcaaaaagt cagatagcac cacatagcag acccgccata aaacgccctg agagcccgtg
      241 acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag gcgcctgtag
      301 tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa
      361 agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg atttgcccga
      421 gaattatccc gcaagaggcc cggcagtcag gtggcacttt tcggggaaat gtgcgcggaa
      481 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      541 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      601 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      661 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      721 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
      781 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
      841 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
      901 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
      961 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1021 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1081 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1141 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1201 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1261 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1321 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1381 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1441 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1501 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1561 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1621 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1681 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1741 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1801 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1861 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1921 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1981 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2041 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2101 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2161 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2221 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2281 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2341 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2401 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2461 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2521 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2581 acacaggaaa cagctatgac catgattacg aattgccggc gatagcttgg ctgcaggtga
     2641 tgattatcag ccagcagaga ttaaggaaaa cagacaggtt tattgagcgc ttatctttcc
     2701 ctttattttt gctgcggtaa gtcgcataaa aaccattctt cataattcaa tccatttact
     2761 atgttatgtt ctgaggggag tgaaaattcc cctaattcga tgaagattct tgctcaattg
     2821 ttatcagcta tgcgccgacc agaacacctt gccgatcagc caaacgtctc ttcaggccac
     2881 tgactagcga taactttccc cacaacggaa caactctcat tgcatgggat cattgggtac
     2941 tgtgggttta gtggttgtaa aaacacctga ccgctatccc tgatcagttt cttgaaggta
     3001 aactcatcac ccccaagtct ggctatgcag aaatcacctg gctcaacagc ctgctcaggg
     3061 tcaacgagaa ttaacattcc gtcaggaaag cttggcttgg agcctgttgg tgcggtcatg
     3121 gaattacctt caacctcaag ccagaatgca gaatcactgg cttttttggt tgtgcttacc
     3181 catctctccg catcaccttt ggtaaaggtt ctaagcttag gtgagaacat ccctgcctga
     3241 acatgagaaa aaacagggta ctcatactca cttctaagtg acggctgcat actaaccgct
     3301 tcatacatct cgtagatttc tctggcgatt gaagggctaa attcttcaac gctaactttg
     3361 agaatttttg caagcaatgc ggcgttataa gcatttaatg cattgatgcc attaaataaa
     3421 gcaccaacgc ctgactgccc catccccatc ttgtctgcga cagattcctg ggataagcca
     3481 agttcatttt tctttttttc ataaattgct ttaaggcgac gtgcgtcctc aagctgctct
     3541 tgtgttaatg gtttcttttt tgtgctcata cgttaaatct atcaccgcaa gggataaata
     3601 tctaacaccg tgcgtgttga ctattttacc tctggcggtg ataatggttg catgtactaa
     3661 ggaggttgta tggaacaacg cataaccctg aaagattatg caatgcgctt tgggcaaacc
     3721 aagacagcta aagatctctc acctaccaaa caatgccccc ctgcaaaaaa taaattcata
     3781 taaaaaacat acagataacc atctgcggtg ataaattatc tctggcggtg ttgacataaa
     3841 taccactggc ggtgatactg agcacatcag caggacgcac tgaccaccat gaaggtgacg
     3901 ctcttaaaaa ttaagccctg aagaagggca gcattcaaag cagaaggctt tggggtgtgt
     3961 gatacgaaac gaagcattgg gatcctaagg aggttaatat tatgcaccat caccatcacc
     4021 atatgacgtc gacgcgtctg cagaagcttc tagaattcga gctcccgggt accatggcat
     4081 gcatcgatag atctcgaggc ctcgcgagct tggcactggc cgtcgtttta caacgtcgtg
     4141 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     4201 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     4261 atggcgaatg gc
//
